Journal:

Article Title: Molecular Basis of Intrinsic Macrolide Resistance in the Mycobacterium tuberculosis Complex

doi: 10.1128/AAC.48.1.143-150.2004

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: The strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Description a Source or reference Strains M. tuberculosis H37Rv ATCC 27294 M. bovis ATCC 19210 M. africanum ATCC 25420 M. microti ATCC 19422 M. bovis BCG Moreau Vaccine strain (Brazil), RD2 region not deleted ATCC 35736 M. bovis BCG Pasteur Vaccine strain, RD2 region deleted, host for DNA cloning no. 1173 P 2 ; Pasteur Institute M. smegmatis mc 2 155 Highly transformable mutant, host for DNA cloning 47 E. coli DH5α Host for DNA cloning Clontech Plasmids pMIP12 Kan r ; high-copy number E. coli-Mycobacterium shuttle vector 28 pIJ43 Amp r ; source of ermE 48 pOS41.33 Amp r , source of srmA 39 pOMV2 Amp r ; PCR (EMT1, EMT2) fragment containing ermMT , cloned into pUC18 This study pOMV16 Kan r ; Bam HI- Kpn I PCR (EMT7, EMT8) fragment containing ermMT , cloned into pMIP12 This study pOMV20 Kan r ; Bam HI- Kpn I PCR (ERME1, ERME2) fragment containing ermE , cloned into pMIP12 This study pOMV30 Kan r ; Bam HI- Kpn I PCR (SRMA1, SRMA2) fragment containing srmA , cloned into pMIP12 This study Open in a separate window a Amp r , ampicillin resistance; Kan r , kanamycin resistance.

Techniques: Plasmid Preparation, Cloning, Mutagenesis, Clone Assay

Journal:

Article Title: Molecular Basis of Intrinsic Macrolide Resistance in the Mycobacterium tuberculosis Complex

doi: 10.1128/AAC.48.1.143-150.2004

Figure Lengend Snippet: MICs of MLS antibiotics against reference strains from the MTC

Article Snippet: The strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Description a Source or reference Strains M. tuberculosis H37Rv ATCC 27294 M. bovis ATCC 19210 M. africanum ATCC 25420 M. microti ATCC 19422 M. bovis BCG Moreau Vaccine strain (Brazil), RD2 region not deleted ATCC 35736 M. bovis BCG Pasteur Vaccine strain, RD2 region deleted, host for DNA cloning no. 1173 P 2 ; Pasteur Institute M. smegmatis mc 2 155 Highly transformable mutant, host for DNA cloning 47 E. coli DH5α Host for DNA cloning Clontech Plasmids pMIP12 Kan r ; high-copy number E. coli-Mycobacterium shuttle vector 28 pIJ43 Amp r ; source of ermE 48 pOS41.33 Amp r , source of srmA 39 pOMV2 Amp r ; PCR (EMT1, EMT2) fragment containing ermMT , cloned into pUC18 This study pOMV16 Kan r ; Bam HI- Kpn I PCR (EMT7, EMT8) fragment containing ermMT , cloned into pMIP12 This study pOMV20 Kan r ; Bam HI- Kpn I PCR (ERME1, ERME2) fragment containing ermE , cloned into pMIP12 This study pOMV30 Kan r ; Bam HI- Kpn I PCR (SRMA1, SRMA2) fragment containing srmA , cloned into pMIP12 This study Open in a separate window a Amp r , ampicillin resistance; Kan r , kanamycin resistance.

Techniques: